Description

Che cos'è la vitamina D?

Vitamin D is a fat-soluble vitamin that is crucial for various bodily functions and involved in thousands of different processes. We call this molecule vitamin D, but its active form, called calciferol, is actually more like a hormone. It exists in two main forms:
Vitamin D3 (cholecalciferol), produced naturally by the body in response to sunlight, and vitamin D2 (ergocalciferol), found in some food sources. Both forms of vitamin D have a similar structure. Vitamin D helps the body use calcium to build strong bones and teeth. It prevents bones from becoming thin, brittle, or misshapen. This is particularly important for children (it prevents rickets) and adults (it prevents osteomalacia). But vitamin D is not just about bones and teeth; it also helps to boost our immune system to fight off infections, keeps our metabolism in check, and even helps our muscles, including the heart, stay strong and stable. Vitamin D is fundamental to mental health. There is sufficient research to demonstrate that a deficiency in vitamin D can lead to symptoms similar to depression.

Why test for Vitamin D?

It is important to test vitamin D levels, as deficiencies are widespread. Testing is important for everyone, especially in densely populated areas where the number of sunshine hours per year is low. A vitamin D deficiency can lead to bone weakness, muscle problems and a weakened immune system. It is important to get tested for vitamin D levels before taking supplements. While beneficial, too much vitamin D can lead to a dangerous buildup of calcium in the blood, known as hypercalcaemia. Testing helps identify whether supplementation is necessary and provides guidance on dosage for optimal

What type of vitamin D do we measure?

Our vitamin D test measures both D3 (cholecalciferol) and D2 (ergocalciferol) separately, ensuring an accurate assessment. The test report provides the combined total vitamin D level for a comprehensive evaluation. We measure 25-hydroxyvitamin D [25(OH)D], the best indicator of your body's vitamin D stores.

How does our test work?

With a single drop of blood, we can measure your vitamin D levels and discover if you're getting enough sun. We've dedicated hours of research to our vitamin D test, perfecting every step to ensure its accuracy. From the moment the sample is taken to the final analysis, we leave nothing to chance. The result? A reliable test, supported by the rigour of laboratory method validation. Our vitamin D test uses advanced techniques such as liquid chromatography-mass spectrometry (LC-MS), which guarantee high precision and sensitivity.
Rigorous sample handling and quality control procedures ensure reliable results on which to base your health decisions.

BloodoTM Vitamin D Test Specifications

Parameter | Specifications

Vitamin D3 | Vitamin D2

Principle of the method
Liquid chromatography coupled with tandem mass spectrometry for the detection of vitamin D3 and D2.

Suitability
For the analysis of human dried blood spots (DBS).

Linearity
Vitamin D3: ≥ 0.99
Vitamin D2: ≥ 0.99

Margin of error
Vitamin D3: 25%
Vitamin D2: 33%

Limit of detection (whole human blood)
Vitamin D3: 14.2 nmol/l
Vitamin D2: 16 nmol/l

Limit of quantification (whole human blood)
Vitamin D3: 23.6 nmol/l
Vitamin D2: 26.6 nmol/l

Linear range (whole human blood)
6.2 – 250 nmol/l

Shipping conditions (DBS test kit)
Ship at ambient temperature.

Storage conditions (DBS test kit)
Store at room temperature.

Shipping conditions (DBS test cards with blood sample)
Send at room temperature. Ship immediately, or by the next day at the latest.
Sample stability limit: 42 giorni.
Maximum sample shipping duration: 35 days.

Conditions for home collection

• • Collect the blood sample at a time convenient for you.
  • Don't take supplements in the Last 24 hours at the test.

• Leave to dry completely DBS card for 3 hours.